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KMID : 0848120080330040149
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International Journal of Oral Biology
2008 Volume.33 No. 4 p.149 ~ p.154
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Porphyromonas Gingivalis Lipopolysaccharide Increases Monocyte Adhesion to Microvascular Endothelium by Induction of Adhesion Molecules
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Kim Su-Ryun
Bae Soo-Kyung
Bae Moon-Kyoung
Park Hyun-Joo
Park Ji-Hyun
Kim Hyo-Sun
Koo Tae-Hyeon
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Abstract
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Porphyromonas gingivalis, a major periodontal pathogen, has been implicated in the initiation and progression of periodontal disease. Endothelial dysfunction (Editor note: Aberrant and dysfunction are somewhat redundant. The authors may want to choose one or the other.) contributes to chronic periodontal inflammation. Using cDNA-representational
difference analysis, we found that P.gingivalis lipopolysaccharide differentially induces a number of genes in human microvascular endothelial cells. Among these upregulated genes, we focused on intercellular adhesion molecule-1 (VCAM-1), which is crucial for leukocyte recruitment during vascular inflammation. P. gingivalis LPS significantly increased the expression of vascular cell adhesion molecule-1 (VCAM-1) as well as ICAM-1. Promoter assays revealed that the transcription of these cell adhesion molecules was mainly regulated by nuclear factor-κB (NF-κB) in endothelial cells. Furthermore, P. gingivalis LPS significantly increased leukocyte adhesiveness to microvascular endothelial cells and to aortic endothelium. Taken together, our results demonstrate that P. gingivalis LPS activates microvascular endothelial cells through NF-κB-dependent expression of cell adhesion molecules.
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KEYWORD
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cDNA representational difference analysis, Cell adhesion molecules, human microvascular endothelial cells, Porphyromonas gingivalis lipopolysaccharide
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